The list ranks Britain’s 100 private companies with the fastest-growing sales over the past three years.
The online travel agent was given the runners-up slot having generated and annual sales rise over three years of 183.9%, culminating in sales of £14.5 million in 2015 having only been founded in 2012.
Alex Francis, chief executive of Loveholidays, said: “We are thrilled to feature so highly in the Sunday Times Virgin Fast Track 100 league table and this is a fantastic accolade for everyone who works for the company.
“Our proprietary cutting-edge technology has helped to drive Loveholidays’ rapid growth since we entered the market just four years ago and enables our customers to shop for holidays in a unique and truly flexible way.
“In an unprecedented trading environment, this award coupled with us becoming a Top 10 Atol-holder earlier in the year is a remarkable achievement and a perfect finish to the year.”
Jonny Marsh, chief operating officer of loveholidays, added: “Our website is the only one in the travel industry with destination-less search technology which puts our customers’ needs at its heart.”
Boutique hotel operator Home Grown Hotels came 76th after reporting an annual sales rise over three years of 57.38%.
This meant £14.2 million of sales were generated in 2015 at the firm’s five Pig hotels, despite only launching as a business in 2011.
The only other travel company to feature in the list cruise travel agent cruise.co.uk which saw annual sales growth of 53.18% in the last three years, generating sales of £36.3 million in 2015.